1B7E
TRANSPOSASE INHIBITOR
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | BRUKER |
| Wavelength(s) | 1.0273,1.0720,1.1201 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 181.800, 71.900, 41.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.195 * |
| Rwork | 0.195 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.630 |
| Data reduction software | DENZO (2000) |
| Data scaling software | SCALEPACK (2000) |
| Phasing software | SOLVE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 * | 0.204 * |
| Total number of observations | 69105 * | |
| Number of reflections | 13121 | |
| <I/σ(I)> | 30.9 | 7.5 |
| Completeness [%] | 94.3 | 87 |
| Redundancy | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 16 (mg/ml) | |
| 2 | 1 | 1 | PEG8000 | 20 (%) | |
| 3 | 1 | 1 | tetraethylammonium sulfate | 100 (mM) | |
| 4 | 1 | 1 | MES | 100 (mM) |
