1B7A
STRUCTURE OF THE PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN FROM BOVINE BRAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.200, 77.160, 107.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.500 - 2.250 |
R-factor | 0.208 * |
Rwork | 0.208 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a44 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.042 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.410 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.057 | 0.122 |
Number of reflections | 16450 | |
<I/σ(I)> | 12.4 | 7.1 |
Completeness [%] | 90.8 | 88.8 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 25-27% PEG8000, 100 MM PHOSPHORYLETHANOLAMINE, pH 4.3 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | dithiothreitol | 2 (mM) | |
4 | 1 | reservoir | PEG8000 | 25-27 (%) | |
5 | 1 | reservoir | phosphorylethanolamine | 100 (mM) |