1B79
N-TERMINAL DOMAIN OF DNA REPLICATION PROTEIN DNAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 118 |
| Collection date | 1998-02-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.300, 66.400, 107.000 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.263 |
| R-free | 0.29300 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.433 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.069 * |
| Number of reflections | 21651 |
| <I/σ(I)> | 9 |
| Completeness [%] | 98.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 20 * | pH 6.9, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 20 (%) | |
| 2 | 1 | drop | cacodylate | 50 (mM) | |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 16 (%) | |
| 5 | 1 | reservoir | cacodylate | 40 (mM) | |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | ethanol | 8 (%) |
