1B77

BUILDING A REPLISOME STRUCTURE FROM INTERACTING PIECES: A SLIDING CLAMP COMPLEXED WITH AN INTERACTION PEPTIDE FROM DNA POLYMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-11
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	65.900, 87.600, 138.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.225
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PRELIMINARY COORDINATE OF T4 GP45 FROM J. KURIYAN
RMSD bond length	0.006
RMSD bond angle	24.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Rmerge	0.070 *
Total number of observations	151555 *
Number of reflections	47361
<I/σ(I)>	6.6
Completeness [%]	94.9
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	20 *	16-18% PEG 8000, 0.2 M CA ACETATE PH7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	PEG8000	8-9 (%(w/v))
3	1	drop	calcium acetate	0.1 (M)
4	1	drop	HEPES	0.1 (M)
5	1	reservoir	PEG8000	16-18 (%(w/v))
6	1	reservoir	calcium acetate	0.2 (M)
7	1	reservoir	HEPES	0.1 (M)