1B76
GLYCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 272 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04-23 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 124.800, 250.900, 105.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.000 - 3.400 |
R-factor | 0.227 * |
Rwork | 0.227 |
R-free | 0.31800 |
Structure solution method | OTHER |
RMSD bond length | 0.014 |
RMSD bond angle | 24.900 * |
Data reduction software | XDS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 3.300 |
Rmerge | 0.095 |
Number of reflections | 20177 |
Completeness [%] | 81.0 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 24 * | pH 8.0, temperature 297K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ETHANOLAMINE | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | (NH4)2SO4 | ||
4 | 1 | 1 | tris-Cl | ||
5 | 1 | 2 | (NH4)2SO4 | ||
6 | 1 | 2 | tris-Cl |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | GlyRS | 0.02 (mM) | |
2 | 1 | drop | ATP | 5 (mM) | or 50 mM ethanolamine |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | ammonium sulfate | 0.4 (M) | |
5 | 1 | drop | Tris-HCl | 50 (mM) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
7 | 1 | reservoir | ammonium sulfate | 1.0-1.4 (M) |