1B6R

N5-CARBOXYAMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE FROM E. COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	280
Detector technology	AREA DETECTOR
Collection date	1998-02-15
Detector	BRUKER
Spacegroup name	C 2 2 21
Unit cell lengths	93.400, 95.200, 120.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.197
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	2.330 *
Data reduction software	SAINT
Data scaling software	XCALIBRE
Refinement software	TNT (5D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.047	0.292
Number of reflections	30332
<I/σ(I)>	24.3	2.4
Completeness [%]	95.6	78.9
Redundancy	3.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	0.525 (M)
3	1	reservoir	HEPPS	100 (mM)