1B6Q

ALANINE 31 PROLINE MUTANT OF ROP PROTEIN

Experimental procedure

Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 2 2 21
Unit cell lengths	30.400, 42.100, 81.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.825 - 1.800
R-factor	0.188 *
Rwork	0.189
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	POLY-ALANINE MODEL FROM PDB ENTRY 1ROP
RMSD bond length	0.007
RMSD bond angle	0.909
Data reduction software	CAD4
Data scaling software	CAD4
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.825	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	5103
<I/σ(I)>	12.4	2.48
Completeness [%]	99.9	99.6
Redundancy	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.3		pH 4.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium acetate	100 (mM)
3	1	drop	ammonium sulfate	100 (mM)
4	1	reservoir	ammonium sulfate	1.6 (M)