1B6I

T4 LYSOZYME MUTANT WITH CYS 54 REPLACED BY THR, CYS 97 REPLACED BY ALA, THR 21 REPLACED BY CYS AND LYS 124 REPLACED BY CYS (C54T,C97A,T21C,K124C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1994-12-15
Spacegroup name	P 32 2 1
Unit cell lengths	60.870, 60.870, 96.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.000 - 1.900
Rwork	0.185
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.600 *
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.074
Number of reflections	14799
<I/σ(I)>	13.5	4.9
Completeness [%]	87.7	74.2
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	5 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	beta-mercaptoehtanol	0.14 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir		0.25 (M)