1B6E

HUMAN CD94

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	91.710, 91.710, 84.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.22
Rwork	0.220
R-free	0.32200
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	27.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.063 *	0.301 *
Number of reflections	6450
<I/σ(I)>	12.5
Completeness [%]	94.5	91.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	15 (%)
2	1	reservoir		100 (mM)
3	1	reservoir	HEPES	50 (mM)