1B6D
BENCE JONES PROTEIN DEL: AN ENTIRE IMMUNOGLOBULIN KAPPA LIGHT-CHAIN DIMER
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.600, 129.200, 86.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.740 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rei 2BJL |
RMSD bond length | 0.009 |
RMSD bond angle | 27.000 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | |
High resolution limit [Å] | 2.740 | 2.740 |
Number of reflections | 12960 | |
Completeness [%] | 97.4 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 18 (%) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
4 | 1 | reservoir | beta-octyl glucoside | 100 (mM) |