1B6C
CRYSTAL STRUCTURE OF THE CYTOPLASMIC DOMAIN OF THE TYPE I TGF-BETA RECEPTOR IN COMPLEX WITH FKBP12
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.580, 81.060, 90.530 |
| Unit cell angles | 86.23, 81.86, 63.92 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.26900 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (0.3C) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 * | 0.243 * |
| Number of reflections | 57740 | |
| <I/σ(I)> | 18 | 6 |
| Completeness [%] | 98.0 | 97.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 20 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.40-1.53 (M) | |
| 3 | 1 | drop | AMP-PNP | 2 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 0.5 (M) |
