1B68

APOLIPOPROTEIN E4 (APOE4), 22K FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	125
Detector technology	CCD
Collection date	1998-03
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	40.210, 53.210, 84.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.2
Rwork	0.200
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bz4
RMSD bond length	0.007
RMSD bond angle	19.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.980
High resolution limit [Å]	1.840	1.840
Rmerge	0.037	0.199
Number of reflections	16493
<I/σ(I)>	27.4	3.7
Completeness [%]	99.1	98.7
Redundancy	4.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2 *	298	WELL : 28% PEG 400, 20MM NAOAC, PH 6.0, 0.1% BME PROTEIN SOLN: 40MM (NH4)H(CO3), 7MG/ML PROTEIN DROPS : WELL/PROTEIN 1/3, ROOM TEMPERATURE, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	22 (%)
2	1	reservoir	sodium acetate	20 (mM)
3	1	reservoir	beta-mercaptoethanol	0.1 (%)