1B62

MUTL COMPLEXED WITH ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	1998-07-01
Detector	RIGAKU RAXIS II
Spacegroup name	I 2 2 2
Unit cell lengths	62.330, 72.630, 190.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.231 *
Rwork	0.231
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	BNL-26192
RMSD bond length	0.006
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.079 *	0.357 *
Number of reflections	24672
<I/σ(I)>	14.4	2
Completeness [%]	97.6	93.6
Redundancy	11.2	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	20 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	100 (mM)
2	1	reservoir		400 (mM)
3	1	reservoir	dithiothreitol	2 (mM)
4	1	reservoir	PEG5000 MME	28 (%)