1B5M

RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-04-18
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	30.000, 30.000, 219.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.22 *
Rwork	0.220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3B5C
RMSD bond length	0.016
RMSD bond angle	2.900
Data reduction software	FRAMBO
Data scaling software	SAINT
Phasing software	MRCHK
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.050
High resolution limit [Å]	2.700	2.700
Rmerge	0.049 *	0.088 *
Number of reflections	3123
<I/σ(I)>	1.5	1
Completeness [%]	96.0	85
Redundancy	2.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION TOGETHER WITH SLOW EVAPORATION USING 20% PEG 8000 IN 0.1M SODIUM CACODYLATE (PH = 6.5 WITH 0.2 M MAGNESIUM ACETATE., vapor diffusion with slow evaporation

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir	sodium cacodylate	0.1 (M)
4	1	reservoir	magnesium acetate	0.2 (M)