1B5D
DCMP Hydroxymethylase from T4 (Intact)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-06-15 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.230, 53.290, 75.860 |
| Unit cell angles | 90.00, 115.54, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.20800 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.868 |
| Data reduction software | MADNESS |
| Data scaling software | FSSCALE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 28632 |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.73 * | Song, S.H., (1999) Acta Crystallogr. D55, 1061. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | dCMP | ||
| 3 | 1 | reservoir | Tris-HCl | 100 (0.9) | |
| 4 | 1 | reservoir | sodium citrate | 0.9 (M) | pH8.73 |
