1B52

OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KTK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 2 A
Unit cell lengths	106.351, 76.798, 69.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.300
Rwork	0.195
R-free	0.26300
Structure solution method	OTHER
Starting model (for MR)	1OLB
RMSD bond length	0.023
RMSD bond angle	0.051
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.083 *	0.415
Number of reflections	25448
<I/σ(I)>		3
Completeness [%]	98.5	90.8
Redundancy	5.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CO-CRYSTALLIZED WITH URANIUM ACETATE, PH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	PEG4000	5-10 (%(w/v))
3	1	reservoir	sodium acetate	50 (mM)
4	1	reservoir	uranyl acetate	1 (mM)