1B4X
ASPARTATE AMINOTRANSFERASE FROM E. COLI, C191S MUTATION, WITH BOUND MALEATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.130, 86.690, 79.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.450 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1asa |
| RMSD bond length | 0.018 |
| RMSD bond angle | 25.510 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.059 | |
| Number of reflections | 17708 | |
| Completeness [%] | 86.0 | |
| Redundancy | 1.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 20 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (mM) | |
| 4 | 1 | drop | EDTA | 5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 40-54 (%) | |
| 6 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 7 | 1 | drop | inhibitor maleate | 5 (mM) |
