1B4N
FORMALDEHYDE FERREDOXIN OXIDOREDUCTASE FROM PYROCOCCUS FURIOSUS, COMPLEXED WITH GLUTARATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.400, 170.500, 180.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aor |
| RMSD bond length | 0.080 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 * | 0.158 * |
| Total number of observations | 193320 * | |
| Number of reflections | 93705 | |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 77.6 | 40.3 |
| Redundancy | 2.1 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | melting-point capillary method * | 5.6 | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 55-65 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | dithionite | 2.0 (mM) | |
| 4 | 1 | 1 | dithiothreitol | 2 (mM) | |
| 5 | 1 | 1 | 0.2 (M) | ||
| 6 | 1 | 2 | glycerol | 30 (%(v/v)) | |
| 7 | 1 | 2 | PEG4000 | 20 (%(w/v)) | |
| 8 | 1 | 2 | sodium citrate | 0.1 (M) | |
| 9 | 1 | 2 | 0.2 (M) |
