1B4L

15 ATMOSPHERE OXYGEN YEAST CU/ZN SUPEROXIDE DISMUTASE ROOM TEMPERATURE (298K) STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-06-15
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	118.900, 118.900, 75.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.25400
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.048 *
Total number of observations	64930 *
Number of reflections	16745
<I/σ(I)>	22	7.4
Completeness [%]	87.7	90.5
Redundancy	3.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.7 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-25 (mg/ml)
2	1	1	phosphate	50 (mM)
3	1	2	ammonium sulfate	2.0-2.2 (M)
4	1	2	Tris-HCl	50 (mM)
5	1	2		50 (mM)
6	1	2		0.001 (%(w/v))