1B4K
High resolution crystal structure of a MG2-dependent 5-aminolevulinic acid dehydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 128.240, 128.240, 86.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.300 - 1.670 |
| Rwork | 0.178 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aw5 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 2.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.054 * | 0.269 * |
| Total number of observations | 719706 * | |
| Number of reflections | 81840 | |
| <I/σ(I)> | 9 | 2.8 |
| Completeness [%] | 98.2 | 94.1 |
| Redundancy | 8.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | drop contained equal volume of protein and reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 5 | 1 | reservoir | glycerol | 12 (%(v/v)) | |
| 6 | 1 | reservoir | isopropanol | 3 (%(v/v)) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
