1B4B

STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-04
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	82.000, 82.000, 67.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.218
Rwork	0.218
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xxc
RMSD bond length	0.005
RMSD bond angle	0.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.052 *	0.216 *
Number of reflections	12146
<I/σ(I)>	18	3
Completeness [%]	99.8	100
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	1	L-arginine	10 (mM)
3	1	1	sodium cacodylate	100 (mM)
4	1	1	2-mercaptoethanol	10 (mM)
5	1	1		100 (mM)
6	1	1		20 (mM)
7	1	1	PEG400	30 (%)