1B4B
STRUCTURE OF THE OLIGOMERIZATION DOMAIN OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS
Experimental procedure
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 82.000, 82.000, 67.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.052 * | 0.216 * |
| Number of reflections | 12146 | |
| <I/σ(I)> | 18 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | |
| 2 | 1 | 1 | L-arginine | 10 (mM) | |
| 3 | 1 | 1 | sodium cacodylate | 100 (mM) | |
| 4 | 1 | 1 | 2-mercaptoethanol | 10 (mM) | |
| 5 | 1 | 1 | 100 (mM) | ||
| 6 | 1 | 1 | 20 (mM) | ||
| 7 | 1 | 1 | PEG400 | 30 (%) |
