1B4A
STRUCTURE OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-12 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.800, 121.900, 227.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xxc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.000 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.054 * | 0.167 * |
Number of reflections | 35454 | |
<I/σ(I)> | 18 | 3 |
Completeness [%] | 98.4 * | 99.5 * |
Redundancy | 5.5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 5 | pH 5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 5 (mg/ml) | |
2 | 1 | 1 | sodium acetate | 100 (mM) | |
3 | 1 | 1 | 2-mercaptoethanol | 10 (mM) | |
4 | 1 | 1 | 0.35 (M) | ||
5 | 1 | 1 | PEG8000 | 16 (%) | |
6 | 1 | 2 | ehthylene glycol | 20 (%) |