1B4A
STRUCTURE OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-12 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.800, 121.900, 227.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.054 * | 0.167 * |
| Number of reflections | 35454 | |
| <I/σ(I)> | 18 | 3 |
| Completeness [%] | 98.4 * | 99.5 * |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 5 | pH 5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | |
| 2 | 1 | 1 | sodium acetate | 100 (mM) | |
| 3 | 1 | 1 | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | 1 | 0.35 (M) | ||
| 5 | 1 | 1 | PEG8000 | 16 (%) | |
| 6 | 1 | 2 | ehthylene glycol | 20 (%) |
