1B49

DCMP HYDROXYMETHYLASE FROM T4 (PHOSPHATE-BOUND)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1998-02-15
Detector	ENRAF-NONIUS
Spacegroup name	C 1 2 1
Unit cell lengths	176.340, 53.270, 75.200
Unit cell angles	90.00, 115.21, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.159
Rwork	0.159
R-free	0.20200
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	0.881
Data reduction software	MADNESS
Data scaling software	FSSCALE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	25579
Completeness [%]	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.73 *		Song, S.H., (1999) Acta Crystallogr. D55, 1061. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	dCMP
3	1	reservoir	Tris-HCl	100 (0.9)
4	1	reservoir	sodium citrate	0.9 (M)	pH8.73