1B3R
RAT LIVER S-ADENOSYLHOMOCYSTEIN HYDROLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.760, 134.480, 102.260 |
| Unit cell angles | 90.00, 114.35, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.197 * |
| Rwork | 0.199 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NAD-BINDING DOMAIN IN FORMATE DEHYDROGENASE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.500 * |
| Data reduction software | DENZO |
| Data scaling software | KUMDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.173 |
| Total number of observations | 280288 * | |
| Number of reflections | 56314 | |
| <I/σ(I)> | 10.1 | 3.4 |
| Completeness [%] | 97.5 | 98 |
| Redundancy | 5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 | 26 * | 50 MM TRIS/HCL (PH 6.8), 6 MM MGCL2, 5% MPD, 15% PEG-6K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 2 | 50 MM TRIS/HCL (PH 6.8) | ||
| 2 | 1 | 2 | 6 MM MGCL2 | ||
| 3 | 1 | 2 | 15% PEG-6K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | PEG6000 | 15 (%(w/v)) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
