1B3J

STRUCTURE OF THE MHC CLASS I HOMOLOG MIC-A, A GAMMADELTA T CELL LIGAND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05
Detector	RIGAKU RAXIS VI
Spacegroup name	F 41 3 2
Unit cell lengths	261.500, 261.500, 261.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9999.000 * - 2.800 *
R-factor	0.253 *
Rwork	0.246
R-free	0.29300 *
Structure solution method	MIR
RMSD bond length	0.014 *
RMSD bond angle	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.050
High resolution limit [Å]	2.800 *	3.000
Rmerge	0.063 *	0.329 *
Total number of observations	130489 *
Number of reflections	19368 *
<I/σ(I)>	23.5	3.2
Completeness [%]	99.7 *	100 *
Redundancy	4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	291 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2SO4
2	1	1	CITRATE BUFFER
3	1	2	(NH4)2SO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	50-55 (%sat)
2	1	reservoir	sodium citrate	50-100 (mM)