1B3D
STROMELYSIN-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 173 |
| Collection date | 1995-11-01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.900, 78.800, 105.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 2.400* |
| Rwork | 0.256 |
| R-free | 0.25600 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.073 |
| Number of reflections | 13990 |
| Completeness [%] | 96.1 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | 20-24% PEG 8000, 2.5% 2-PROPANOL, 10 MM CACL2 0.1 M TRIS-HCL BUFFER, PH 7.5-8.5, pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 20-24 (%(w/v)) | precipitant |
| 3 | 1 | drop | 2-propanol | 2.5 (%(v/v)) | precipitant |
| 4 | 1 | drop | 10 (mM) | precipitant | |
| 5 | 1 | drop | Tris-HCl | 0.1 (M) | precipitant |
