1B3B
THERMOTOGA MARITIMA GLUTAMATE DEHYDROGENASE MUTANT N97D, G376K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 286 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 145.100, 145.100, 272.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 3.100 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.543 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.140 |
High resolution limit [Å] | 3.100 | 3.000 |
Rmerge | 0.096 | |
Total number of observations | 151200 * | |
Number of reflections | 53329 | |
Completeness [%] | 94.0 | 60 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 20 * | 6% POLYETHYLENE GLYCOLE, 120 MM AMMONIUM ACETATE, 50 MM BIS-TRIS PROPANE PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | Bis-Tris propane | 50 (mM) | |
4 | 1 | reservoir | ammonium acetate | 120 (mM) | |
5 | 1 | reservoir | PEG4000 | 3 (%) |