1B38

HUMAN CYCLIN-DEPENDENT KINASE 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-21
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	53.333, 71.122, 72.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.180
R-free	0.25000
Structure solution method	OTHER
Starting model (for MR)	UNPUBLISHED
RMSD bond length	0.016
RMSD bond angle	0.042
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.092	0.390
Total number of observations	70729 *
Number of reflections	18076
<I/σ(I)>	11	2.26
Completeness [%]	95.2	96.8
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4		PROTEIN AT 10 MG/ML IN 10MM HEPES/HCL PH7.4, 15MM NACL WELL BUFFER CONTAINING 50MM AMMONIUM ACETATE, 12% PEG 3350, 100MM HEPES/HCL PH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		15 (mM)
3	1	drop	HEPES	10 (mM)
4	1	reservoir	PEG3350	10-15 (%)
5	1	reservoir	ammonium acetate	50 (mM)
6	1	reservoir	HEPES	0.1 (M)