1B35
CRICKET PARALYSIS VIRUS (CRPV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-07 |
| Detector | FUJI |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 330.000, 334.000, 395.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2plv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.128 * | 0.015 |
| Total number of observations | 312272 * | |
| Number of reflections | 210945 | |
| <I/σ(I)> | 11 | 5 |
| Completeness [%] | 25.2 | 9.2 |
| Redundancy | 1.23 | 1.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | VIRUS STORED AT 10MG/ML IN 200MM NAH2PO4, PH7.2. WELL SOLUTION CONSISTED OF 8% (W/V) MPEG 5K, 50MM LI2SO4, 50MM MES, PH6.0. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | virus | 10 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 200 (mM) | |
| 3 | 1 | reservoir | mPEG5000 | 8 (%(w/v)) | |
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | MES | 50 (mM) |
