1B2W

COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	286
Detector technology	AREA DETECTOR
Collection date	1994-07-01
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	69.100, 74.600, 104.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.188 *
Rwork	0.188
R-free	0.33100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dfb
RMSD bond length	0.009
RMSD bond angle	27.200 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.960
High resolution limit [Å]	2.790	2.790
Rmerge	0.093	0.410
Number of reflections	11491
<I/σ(I)>	8.3	1
Completeness [%]	67.2 *	0.28
Redundancy	2.2	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.2	18-20 *	6.6% PEG 8000(W/V), IN 0.05 M NACL AND 0.05% SODIUM AZIDE., pH 6.2

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	2	6.6% PEG 8000(W/V)
2	1	2	0.05 M NACL
3	1	2	0.05% SODIUM AZIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35-45 (mg/ml)
2	1	reservoir	PEG8000	6.6 (%(w/v))
3	1	reservoir		0.05 (M)
4	1	reservoir	sodium azide	0.05 (%)