1B2U
STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.230, 43.020, 83.470 |
| Unit cell angles | 90.00, 110.70, 90.00 |
Refinement procedure
| Resolution | 21.300 - 2.100 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.27600 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1brs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 * | 0.298 |
| Number of reflections | 32841 | |
| <I/σ(I)> | 14.45 | 4 |
| Completeness [%] | 98.9 | 99.7 * |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | NH4 SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 18-24 (%(w/v)) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
