1B2O
CLOSTRIDIUM PASTEURIANUM RUBREDOXIN G10VG43A MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07-01 |
Detector | RIGAKU RU200 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.850, 61.850, 80.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.194 * |
Rwork | 0.194 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5rxn |
RMSD bond length | 0.014 |
RMSD bond angle | 0.026 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 1.900 | 1.940 |
Rmerge | 0.092 | 0.280 |
Total number of observations | 54730 * | |
Number of reflections | 12136 | |
<I/σ(I)> | 10.9 | 3.7 |
Completeness [%] | 94.0 | 96 * |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | 277 * | PROTEIN WAS CRYSTALLISED FROM 70% SATURATED AMMONIUM SULFATE IN SODIUM ACETATE BUFFER (50 MM) AT PH 5.0. |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 2 | 70% SATURATED AMMONIUM SULFATE | ||
2 | 1 | 2 | SODIUM ACETATE BUFFER (50 MM) AT PH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-20 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 2-35 (%) | |
3 | 1 | drop | sodium acetate | 50 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 50-70 (%) | |
5 | 1 | reservoir | sodium acetate | 50 (mM) |