1B2H

Oligo-Peptide Binding Protein Complexed with Lysyl-Ornithyl-Lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Spacegroup name	P 21 21 21
Unit cell lengths	109.659, 76.124, 70.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.180
R-free	0.21800 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.029 *
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Rmerge	0.087	0.144 *
Number of reflections	46360
<I/σ(I)>	6
Completeness [%]	98.5	90.4 *
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	PEG4000	7 (%)
3	1	reservoir	uranyl acetate	1 (mM)
4	1	reservoir	sodium acetate	50 (mM)