1B2D
PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.35 COORDINATES)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 287 |
Spacegroup name | I 21 3 |
Unit cell lengths | 78.811, 78.811, 78.811 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.22900 |
Structure solution method | OTHER |
RMSD bond length | 0.006 |
RMSD bond angle | 22.490 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.000 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.086 * |
Number of reflections | 9068 |
Completeness [%] | 99.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 9.2 * | pH 6.35 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium phosphate | 0.05 (M) | |
2 | 1 | 1 | sodium EDTA | 0.001 (M) | pH11 |
3 | 1 | 1 | protein | 18 (mg/ml) | |
4 | 1 | 2 | sodium phosphate | 0.15 (M) | |
5 | 1 | 2 | sodium EDTA | 0.001 (M) | pH9.2 |