1B1Y
SEVENFOLD MUTANT OF BARLEY BETA-AMYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 291 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-04-01 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.110, 72.110, 250.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1btc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 24.770 * |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.086 * |
| Total number of observations | 122221 * |
| Number of reflections | 22631 |
| Completeness [%] | 94.3 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 18 * | HUNGING DROP VAPOR DIFFUSION AGAINST 0.1 M PIPES PH 7.1, 0.1 M MGAC2 AND 14% PEG 6000 WITH A PROTEIN CONCENTRATION OF 3 MG/ML., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | drop | PIPES | 50 (mM) | |
| 3 | 1 | drop | magnesium acetate | 0.2 (M) | |
| 4 | 1 | drop | PEG6000 | 7 (%) | |
| 5 | 1 | drop | PMSF | 1 (mM) | |
| 6 | 1 | drop | 0.01 (%(w/v)) | ||
| 7 | 1 | reservoir | PIPES | 0.1 (M) | |
| 8 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 9 | 1 | reservoir | PEG6000 | 14 (%) |
