Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-23 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.380, 54.480, 40.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.219 * |
Rwork | 0.222 |
R-free | 0.27700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bip MODEL 20 |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE ((CCP4)) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 * | 2.280 |
High resolution limit [Å] | 2.200 | 2.700 * |
Rmerge | 0.072 * | 0.162 * |
Total number of observations | 57852 * | |
Number of reflections | 5094 * | |
<I/σ(I)> | 11.2 | 2.27 |
Completeness [%] | 91.0 * | 83.5 * |
Redundancy | 12.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 8 | 1.15M AMMONIUM SULFATE, 0.2M AMMONIUM PHOSPHATE PH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium phosphate | 20 (mM) | pH8.0 |
2 | 1 | 1 | ammonium sulfate | 0.3 (M) | |
3 | 1 | 1 | protein | 10-15 (mg/ml) | |
4 | 1 | 2 | ammonium sulfate | 1.15 (M) | |
5 | 1 | 2 | sodium phosphate | 0.4 (M) | pH8.0 |