1B1J
CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN VARIANT H13A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 289 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 67.170, 104.470, 38.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.800 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.242 |
| Total number of observations | 21688 * | |
| Number of reflections | 5493 | |
| <I/σ(I)> | 4.7 | 1.3 |
| Completeness [%] | 58.3 | 31.8 |
| Redundancy | 3.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 16 * | pH 7.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | HEPES | ||
| 5 | 1 | 2 | (NH4)2SO4 | ||
| 6 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1 (M) | |
| 4 | 1 | drop | PEG400 | 1 (%) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | |
| 6 | 1 | reservoir | PEG400 | 2 (%) |
