1B1I
CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.650, 37.810, 42.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.378 |
| Total number of observations | 104187 * | |
| Number of reflections | 11357 | |
| <I/σ(I)> | 9.3 | 2.6 |
| Completeness [%] | 87.8 | 72.1 |
| Redundancy | 7.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.2 * | 16 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 10 (mM) | |
| 3 | 1 | drop | sodium potassium tartrate | 0.1 (M) | |
| 4 | 1 | drop | PEG6000 | 5 (%) | |
| 5 | 1 | reservoir | sodium citrate | 20 (mM) | |
| 6 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
| 7 | 1 | reservoir | PEG6000 | 10 (%) |
