1B1H

OLIGO-PEPTIDE BINDING PROTEIN/TRIPEPTIDE (LYS HPE LYS) COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	120
Spacegroup name	P 21 21 21
Unit cell lengths	109.722, 75.953, 70.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.191 *
Rwork	0.190
R-free	0.22000
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	0.025
Data reduction software	DENZO
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.077	0.126 *
Total number of observations	238371 *
Number of reflections	54469
<I/σ(I)>	7
Completeness [%]	98.7 *	95.4 *
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		pH 5.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	PEG4000	7 (%)
3	1	reservoir	uranyl acetate	1 (mM)
4	1	reservoir	sodium acetate	50 (mM)