1B1E
CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN VARIANT K40Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 289 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.660, 120.030, 37.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ang |
RMSD bond length | 0.010 |
RMSD bond angle | 28.000 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.248 |
Total number of observations | 104187 * | |
Number of reflections | 13155 | |
<I/σ(I)> | 4.9 | 1.3 |
Completeness [%] | 98.3 | 94.9 |
Redundancy | 7.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.2 | 16 * | pH 5.2, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CITRATE | ||
2 | 1 | 1 | SODIUM POTASIUM TARTARATE | ||
3 | 1 | 1 | PEG 4000 | ||
4 | 1 | 2 | SODIUM CITRATE | ||
5 | 1 | 2 | SODIUM POTASIUM TARTARATE | ||
6 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium citrate | 10 (mM) | |
3 | 1 | drop | sodium potassium tartrate | 0.1 (M) | |
4 | 1 | drop | PEG6000 | 5 (%) | |
5 | 1 | reservoir | sodium citrate | 20 (mM) | |
6 | 1 | reservoir | sodium potassium tartrate | 0.2 (M) | |
7 | 1 | reservoir | PEG6000 | 10 (%) |