1B1B
IRON DEPENDENT REGULATOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 |
| Unit cell lengths | 92.600, 92.600, 63.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.28 |
| Rwork | 0.280 |
| R-free | 0.36000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AMORE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 * | 0.450 * |
| Total number of observations | 147166 * | |
| Number of reflections | 9277 | |
| <I/σ(I)> | 19.6 | 4.1 |
| Completeness [%] | 95.2 | 92.6 * |
| Redundancy | 15 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | protein solution is mixed in a 2:1 ratio with well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 3 | 1 | reservoir | zinc acetate | 10 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 5 | 1 | reservoir | glycerol | 10 (%(w/v)) |
