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1B17

PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 5.00 COORDINATES)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Temperature [K]287
Spacegroup nameI 21 3
Unit cell lengths78.712, 78.712, 78.712
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 1.700
Rwork0.184
R-free0.21990

*

Structure solution methodOTHER
RMSD bond length0.006
RMSD bond angle21.890

*

Phasing softwareX-PLOR
Refinement softwareX-PLOR (3.843)
Data quality characteristics
 Overall
Low resolution limit [Å]55.000
High resolution limit [Å]1.700
Rmerge0.083

*

Number of reflections9026
Completeness [%]99.3

*

Redundancy3.86
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Microdialysis

*

9.2

*

pH 5.00
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
1121M SODIUM SULFATE SOLUTION (PH 5.00)23
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
111sodium phosphate0.05 (M)
211sodium EDTA0.001 (M)pH11
311protein18 (mg/ml)
412sodium phosphate0.15 (M)
512sodium EDTA0.001 (M)pH9.2

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PDB entries from 2024-10-30

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