1B0Z

The crystal structure of phosphoglucose isomerase-an enzyme with autocrine motility factor activity in tumor cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1997-07-25
Detector	RIGAKU RAXIS II
Spacegroup name	I 2 2 2
Unit cell lengths	75.100, 93.730, 171.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.185
R-free	0.25800
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	1.340
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.700	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.360
Number of reflections	24024
<I/σ(I)>	12	3.2
Completeness [%]	93.9	83.4
Redundancy	4.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		CRYSTALS WERE GROWN BY HANGING DROP VAPOR DIFFUSION FROM 4-UL DROPLETS OF PROTEIN SOLUTION (15 MG/ML) IN 50 MM PHOSPHATE BUFFER (PH7.0) AND 0.2 M AMMONIUM PHOSPHATE AGAINST A RESERVOIR OF THE ABOVE BUFFER CONTAINING 0.4M AMMONIUM PHOSPHATE., VAPOR DIFFUSION, HANGING DROP