1B0Y
MUTANT H42Q OF HIPIP FROM CHROMATIUM VINOSUM AT 0.93A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-24 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.681, 41.675, 41.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 0.930 |
R-factor | 0.136 * |
R-free | 0.20460 |
Structure solution method | DIRECT METHODS |
RMSD bond length | 0.014 |
RMSD bond angle | 0.034 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 0.950 |
High resolution limit [Å] | 0.930 | 0.930 |
Rmerge | 0.073 | 0.297 * |
Total number of observations | 402616 * | |
Number of reflections | 39854 | |
<I/σ(I)> | 11.5 | 3.1 |
Completeness [%] | 93.0 | 88.9 |
Redundancy | 10.1 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.3 | 277 * | 2M AMMONIUM SULPHATE, 40 MM TRI 180 MM KCL, pH 5.3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 90 (mM) | ||
4 | 1 | drop | ammonium sulfate | 1 (M) | |
5 | 1 | reservoir | ammonium sulfate | 2 (M) |