1B0X

THE CRYSTAL STRUCTURE OF AN EPH RECEPTOR SAM DOMAIN REVEALS A MECHANISM FOR MODULAR DIMERIZATION.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	90
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 64
Unit cell lengths	77.140, 77.140, 24.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.229 *
Rwork	0.229
R-free	0.27800
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	1.380
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.004 *
Total number of observations	17787 *
Number of reflections	5663 *
Completeness [%]	97.5
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	100 (mg/ml)
2	1	drop	HEPES	7 (mM)
3	1	reservoir	cacodylate	100 (mM)
4	1	reservoir	PEG8000	7 (%(w/v))
5	1	reservoir	ethylene glycol	20 (%(v/v))