1B0W
Structural comparison of amyloidogenic light chain dimer in two crystal forms with nonamyloidogenic counterparts
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-02-01 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.040, 142.110, 77.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| Rwork | 0.226 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bre |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.840 |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 2.000 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.071 | 0.218 |
| Number of reflections | 35142 | |
| <I/σ(I)> | 7.5 | 1.92 |
| Completeness [%] | 76.0 | 56 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | CRYSTALS OF BRE (20MG/ML IN 0.1M TRIS BUFFER, PH 7) WERE GROWN AT RT IN HANGING-DROPS USING 2.5M AMMONIUM SULFATE IN 0.1M CITRATE BUFFER (PH 5.5)., pH 6.5, VAPOR DIFFUSION, HANGING DROP |
