1B0V
I40N MUTANT OF AZOTOBACTER VINELANDII FDI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 42.000, 55.300, 62.300 |
Unit cell angles | 78.10, 85.20, 71.90 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.209 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fd1 |
RMSD bond length | 0.020 |
RMSD bond angle | 3.830 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.165 * | 0.430 * |
Total number of observations | 17912 * | |
Number of reflections | 11182 * | |
<I/σ(I)> | 3 | 1.2 |
Completeness [%] | 87.3 | 90 * |
Redundancy | 1.6 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 2 * | Shen, B., (1994) J. Biol. Chem., 269, 8564. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-10 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 0.5 (M) | |
3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
4 | 1 | reservoir | ammonium sulfate | 3.5 (M) |