1B0U
ATP-BINDING SUBUNIT OF THE HISTIDINE PERMEASE FROM SALMONELLA TYPHIMURIUM
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-15 |
| Detector | ADSC |
| Wavelength(s) | 1.000, 0.9800, 0.97977, 0.9686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.809, 68.809, 148.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 23.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.266 |
| Number of reflections | 56098 | |
| <I/σ(I)> | 10.4 | 3.8 |
| Completeness [%] | 97.0 | 88.1 |
| Redundancy | 3.7 | 2.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sparse matrix method * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 10 | 1 | 2 | ATP | 10 (mM) | |
| 2 | 1 | 1 | glycerol | 20 (%) | |
| 3 | 1 | 1 | EDTA | 2 (mM) | |
| 4 | 1 | 1 | imidazol | 50 (mM) | |
| 5 | 1 | 1 | HEPES | 0.1 (M) | |
| 6 | 1 | 1 | ATP | 10 (mM) | |
| 7 | 1 | 2 | PEG6000 | 20 (%) | |
| 8 | 1 | 2 | 1.2 (M) | ||
| 9 | 1 | 2 | HEPES | 0.1 (M) |
