1B0O
BOVINE BETA-LACTOGLOBULIN COMPLEXED WITH PALMITATE, LATTICE Z
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.480, 53.480, 111.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.2049 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1beb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.366 |
| Total number of observations | 97142 * | |
| Number of reflections | 6888 | |
| <I/σ(I)> | 21.2 | 3.95 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.1 | 8.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | a molar ratio of 10/protein dimer | |
| 2 | 1 | drop | palmitic acid | 100 (mM) | |
| 3 | 1 | drop | ethanol | ||
| 4 | 1 | reservoir | sodium citrate | 1.34 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) |
