1B0N
SINR PROTEIN/SINI PROTEIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.900, 60.900, 87.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.199 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | Agrovata |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 * | 0.149 * |
| Total number of observations | 45969 * | |
| Number of reflections | 15070 | |
| <I/σ(I)> | 13.4 | 4.8 |
| Completeness [%] | 98.7 | 94.7 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 18 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | zinc acetate | 300 (mM) | |
| 2 | 1 | reservoir | MES | 50 (mM) | |
| 3 | 1 | reservoir | mPEG2000 | 1-4 (%) | |
| 4 | 1 | reservoir | sodium chloride | 200 (mM) |
